Acetonitrile-assisted enzymatic digestion can facilitate the Bottom-up identification of proteins of cancer origin

0501150 - ÚIACH 2020 RIV US eng J - Článek v odborném periodiku
Laštovičková, Markéta - Bobál, P. - Strouhalová, Dana - Bobálová, Janette
Acetonitrile-assisted enzymatic digestion can facilitate the Bottom-up identification of proteins of cancer origin.
Analytical Biochemistry. Roč. 570, APR (2019), s. 1-4. ISSN 0003-2697. E-ISSN 1096-0309
Grant CEP: GA MŠMT(CZ) 8F17003
Institucionální podpora: RVO:68081715
Klíčová slova: breast cancer * improvement of digestion * proteins
Obor OECD: Analytical chemistry
Impakt faktor: 2.877, rok: 2019
Způsob publikování: Omezený přístup

In-gel trypsin digestion protocol using aqueous/acetonitrile solvent system facilitates MS analysis and maximize the number of identified proteins from biological samples. The procedure increased the number of matched peptides, and allowed the identification of more proteins from breast cancer cells with higher coverage than the common digestion protocol.
Trvalý link: http://hdl.handle.net/11104/0293142