Critical defects in cryopreserved cell nuclei: DNA structure changes

0500594 - FZÚ 2019 RIV US eng A - Abstrakt
Falk, Martin - Falková, Iva - Pagáčová, Eva - Kopečná, Olga - Bačíková, Alena - Šimek, Daniel - Golan, Martin - Klejdus, B. - Varga, K. - Teplá, O. - Kratochvílová, Irena
Critical defects in cryopreserved cell nuclei: DNA structure changes.
Cryobiology. Elsevier. Roč. 85, č. 12 (2018), s. 135-135. ISSN 0011-2240. E-ISSN 1090-2392.
[CRYO 2018 (55th Annual Meeting of the Society for Cryobiology). 10.07.2018-13.07.2018, Madrid]
Grant CEP: GA MŠMT EF16_013/0001406; GA MŠMT(CZ) LO1409
Grant ostatní: OP VVV - SAFMAT(XE) CZ.02.1.01/0.0/0.0/16_013/0001406
Institucionální podpora: RVO:68378271 ; RVO:68081707
Klíčová slova: cryopreservation * cell replication * replication fork collapse * double-strand break * chromatin condensation * DMSO
Obor OECD: Biophysics
https://www.sciencedirect.com/science/article/pii/S0011224018303948

We showed that frozen/thawed S-phase cells have collapsed replication forks, regardless of the used cryoprotectant. This may have serious implications for assisted reproduction. We also identified changes in higher-order chromatin structure associated with the freeze-thaw process, and correlated them with the viability of frozen and thawed cells. Both in replicating and non-replicating cells, freezing and thawing altered the chromatin structure in a cryoprotectant-dependent manner. Our results suggest that chromatin condensation plays a positive role in cell survival.
Trvalý link: http://hdl.handle.net/11104/0292680