Protein pattern and transglycosylation activity of Geobacillus kaustophilus.

0500180 - MBÚ 2019 RIV IN eng J - Článek v odborném periodiku
Mikulík, Karel - Bezoušková, Silvia - Melčová, M. - Zídková, J.
Protein pattern and transglycosylation activity of Geobacillus kaustophilus.
Current Trends in Microbiology. Roč. 12, DEZ (2018), s. 45-53. ISSN 0972-7736
Institucionální podpora: RVO:61388971
Klíčová slova: transglycosylation * floxuridine * Geobacillus kaustophilus
Obor OECD: Microbiology

Thermophile bacterium Geobacillus kaustophilus was able to synthesize 5-fluoro-2´-deoxyuridine (floxuridine) to 52 mg.L 1.h 1 using whole cells. The presence of 1 mmol.L 1 ZnCl2 enhanced transglycosylation reaction. The reaction was also enhanced when 2-deoxyuridine (2’dur) was, even partially, replaced with 5-fluoro-2´-deoxyriboside (5’Fdri). The highest production of floxuridine was observed at the 5’Fdri to 2’dur ratio of 5:1, i.e. when 5’Fdri was in great excess. G. kaustophilus can accept both 6-oxo- and 6 aminopurine nucleotides as substrates, but oxo-nucleotides are more efficient substrates than 2´-amino modified compounds. Mass spectrometric analyses resulted in the identification of 23 proteins representing metabolic enzymes, and purine nucleotide phosphorylase DeoD subunit. This strain can be used as a catalyst in the biosynthesis of 2´ fluorinate nucleotides.


Trvalý link: http://hdl.handle.net/11104/0292302