Method of determining the activity of enzymes converting cytosine derivatives to uracil derivatives in cells, tissues and organisms

0499716 - ÚOCHB 2019 RIV eng P - Patentový dokument
Koberna, K. - Ligasová, A. - Rosenberg, Ivan - Liboska, Radek - Páv, Ondřej - Kvapil, P. - Fišer, J.
Method of determining the activity of enzymes converting cytosine derivatives to uracil derivatives in cells, tissues and organisms.
2018. Vlastník: Univerzita Palackého v Olomouci - Ústav organické chemie a biochemie AV ČR, v. v. i. - Institute of Applied Biotechnologies, a. s. Datum udělení patentu: 17.10.2018. Číslo patentu: EP3186387
Grant CEP: GA MŠMT(CZ) LO1304; GA TA ČR TA03010598
Institucionální podpora: RVO:61388963
Klíčová slova: cytidine deaminase * dCMP deaminase * click reaction * substrate deamination
Obor OECD: Biochemistry and molecular biology
https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=EP&NR=3186387B1&KC=B1&FT=D&ND=4&date=20181017&DB=&locale=en_EP#

The developed method allows a quick determination of the activity of enzymes transforming cytosine derivatives into uracil derivatives in a sample of tissues, cells or in entire organisms, using substrate with clickable moiety. Substrate is deaminated and subsequently determined using a reaction with a specific antibody or by click reaction.
Trvalý link: http://hdl.handle.net/11104/0292050