Nanostructures in Biomedicine: Their Regenerative and toxic properties

0499259 - ÚEM 2019 RIV CZ eng M - Část monografie knihy
Brzicová, Táňa - Vrbová, Kristýna - Topinka, Jan - Rössner ml., Pavel
Cytotoxicity of Nanomaterials Applicable in Restoration and Conservation.
Nanostructures in Biomedicine: Their Regenerative and toxic properties. Praha 3: Proxima Tisk, s.r.o., 2018 - (Pamula, E.), s. 21-28. ISBN 978-80-906655-7-6
Grant CEP: GA MŠMT(CZ) LO1508
Institucionální podpora: RVO:68378041
Klíčová slova: nanotechnology * cytotoxicity * restoration
Obor OECD: Cell biology

Nanotechnologies represent new promising approach for conservation and preservation of cultural heritage. Nanomaterialshave been shown to improve efficacy, durability and quality of the restoration due to their unique properties that can be achieved and tuned by controlling particle physico-chemical characteristics. The same characteristics, however, affect the way how nanomaterials interact with biological systems and may potentially underlie their enhanced or unexpected toxicity. In this pilot study, we compared the toxic potential of representatives of three of the most common oxide materials applicable in
restoration: TiO2 (standard and purified P25, a mixture of prevailing anatase with rutile crystalline modifications), SiO2 (bare A200, and R805, R9200 as coated forms of A200), and ZnO. Using two in vitro cytotoxicity assays, WST-1 and LDH, evaluating metabolic activity and cell membrane integrity, respectively, we preliminary ranked the tested substances according to their cytotoxic potential, which may be used for their prioritization for further testing and applications. After 24 h exposure, a dose-dependent decrease in cell viability was only detected in ZnO nanoparticles and uncoated silica A200). Hydrophobic coated silicas (R805 and R9200) and TiO2 nanoparticles (purified and unpurified P25) did not exhibit cytotoxic effects up to the highest tested concentration of 250 μg/mL. The tested nanomaterials were ranked according to their cytotoxic potential expressed as IC50. Toxicological data related to the physico-chemical characteristics will be applicable in developing both more efficient and safer nano-based products for restoration and conservation.

Trvalý link: http://hdl.handle.net/11104/0293439