Rapid fabrication of microextraction devices for direct determination of basic drugs in dried blood spots

0499020 - ÚIACH 2019 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Ryšavá, Lenka - Přikryl, Jan - Dvořák, Miloš - Malá, Zdeňka - Kubáň, Pavel
Rapid fabrication of microextraction devices for direct determination of basic drugs in dried blood spots.
CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2018 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.; Přikryl, J.), s. 257-260. ISBN 978-80-904959-5-1.
[CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./. Brno (CZ), 15.10.2018-17.10.2018]
Grant CEP: GA ČR(CZ) GA18-13135S
Institucionální podpora: RVO:68081715
Klíčová slova: supported liquid membrane (SLM) * capillary electrophoresis * microextraction devices
Obor OECD: Analytical chemistry
http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf

A 3D-printed cutter was designed for rapid fabrication of supported liquid membrane (SLM) extraction devices compatible with injection system of commercial capillary electrophoresis (CE) instrument. Low cost and single use microextraction devices were fabricated from polypropylene micropipette tips with high precision (dimension variances ≤ 3% RSD). Practical applicability of the microextraction devices was examined by in-line coupling of SLM extractions to CE for direct analysis of four model basic drugs in dried blood spot extracts. Excellent repeatability of the hyphenated technique was achieved (RSD ≤ 7.6%), transfer of analytes across the SLM ranged from 23 to 73% and limits of detection were as low as 0.07 μg/mL.
Trvalý link: http://hdl.handle.net/11104/0291313