A Microfluidic devide for monitoring cell migration

0498642 - ÚIACH 2019 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Ledvina, Vojtěch - Klepárník, Karel - Legartová, Soňa - Bártová, Eva
A Microfluidic devide for monitoring cell migration.
CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2018 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.; Přikryl, J.), s. 210-213. ISBN 978-80-904959-5-1.
[CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./. Brno (CZ), 15.10.2018-17.10.2018]
Grant CEP: GA ČR(CZ) GA17-01995S
Institucionální podpora: RVO:68081715 ; RVO:68081707
Klíčová slova: microfluidic device * cell migration * Premo FUCCI Cell Cycle Sensor
Obor OECD: Analytical chemistry; Cell biology (BFU-R)
http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf

A polydimethylsiloxane microfluidic device for monitoring directed cell migration was developed. The device comprises a main channel barred by pillar arrays with decreasing spacing and utilizes chemotactic movement of cells in the direction of increasing gradient of fetal bovine serum. HeLa cell line transfected with Premo FUCCI Cell Cycle Sensor was used as a model organism. The cells were imaged for several days using a scanning confocal microscope and the rate of migration in different cell cycle phases was evaluated.
Trvalý link: http://hdl.handle.net/11104/0290940