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Evaluation of the 33 synthesized nitrophenol-based markers of isoelectic point
- 1.0498544 - ÚIACH 2019 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Duša, Filip - Moravcová, Dana - Šlais, Karel
Evaluation of the 33 synthesized nitrophenol-based markers of isoelectic point.
CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2018 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.; Přikryl, J.), s. 132-135. ISBN 978-80-904959-5-1.
[CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./. Brno (CZ), 15.10.2018-17.10.2018]
Grant CEP: GA ČR(CZ) GA16-03749S; GA MV VI20172020069
Institucionální podpora: RVO:68081715
Klíčová slova: nitrophenol-based markers * isoelectic point * capillary isoelectric focusing
Obor OECD: Analytical chemistry
http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf
33 nitrophenol-based compounds, which absorb at ultraviolet as well as visible wavelengths, were selected as candidates for marking isoelectric points (pI) over the pH range from 3 to 10. The evaluation and selection of the candidate structures included structure-based calculations of acid-base properties. The selected compounds were further tested experimentally using capillary isoelectric focusing. The experimental analysis excluded two compounds due to producing double peaks during the analysis. Overall, 31 novel compounds were identified as suitable pI markers.
Trvalý link: http://hdl.handle.net/11104/0290886
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