TbUTP10, a protein involved in early stages of pre-18S rRNA processing in Trypanosoma brucei

0498156 - BC 2019 RIV NL eng J - Článek v odborném periodiku
Faktorová, Drahomíra - Bar, A. - Hashimi, Hassan - McKenney, K. M. - Horák, Aleš - Schnaufer, A. - Rubio, M.A.T. - Alfonzo, J. D. - Lukeš, Julius
TbUTP10, a protein involved in early stages of pre-18S rRNA processing in Trypanosoma brucei.
Molecular and Biochemical Parasitology. Roč. 225, OCT (2018), s. 84-93. ISSN 0166-6851. E-ISSN 1872-9428
Grant CEP: GA ČR(CZ) GA16-18699S; GA ČR GA17-24036S; GA MŠMT LL1601; GA MŠMT(CZ) EF16_019/0000759
Institucionální podpora: RVO:60077344
Klíčová slova: Trypanosoma * Ribosomal RNA * Pre-18S rRNA processing * utp10 * U3 snoRNA
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 2.158, rok: 2018

Ribosome biosynthesis, best studied in opisthokonts, is a highly complex process involving numerous protein and RNA factors. Yet, very little is known about the early stages of pre-18S rRNA processing even in these model organisms, let alone the conservation of this mechanism in other eukaryotes. Here we extend our knowledge of this process by identifying and characterizing the essential protein TbUTP10, a homolog of yeast U3 small nucleolar RNA-associated protein 10 UTP10 (HEATR1 in human), in the excavate parasitic protist Trypanosoma brucei. We show that TbUTP10 localizes to the nucleolus and that its ablation by RNAi knock-down in two different T. brucei life cycle stages results in similar phenotypes: a disruption of pre-18S rRNA processing, exemplified by the accumulation of rRNA precursors, a reduction of mature 18S rRNA, and also a decrease in the level of U3 snoRNA. Moreover, polysome profiles of the RNAi-induced knock-down cells show a complete disappearance of the 40S ribosomal subunit, and a prominent accumulation of the 60S large ribosomal subunit, reflecting impaired ribosome assembly. Thus, TbUTP10 is an important protein in the processing of 18S rRNA.
Trvalý link: http://hdl.handle.net/11104/0290562