Development of microsatellite loci in zoonotic tapeworm Dibothriocephalus latus (Linnaeus, 1758), Luhe, 1899 (syn. Diphyllobothrium latum) using microsatellite library screening

0497528 - BC 2019 RIV NL eng J - Článek v odborném periodiku
Bazsalovicsová, E. - Koleničová, A. - Kralová-Hromadová, I. - Minárik, G. - Šoltys, K. - Kuchta, Roman - Štefka, Jan
Development of microsatellite loci in zoonotic tapeworm Dibothriocephalus latus (Linnaeus, 1758), Luhe, 1899 (syn. Diphyllobothrium latum) using microsatellite library screening.
Molecular and Biochemical Parasitology. Roč. 225, OCT (2018), s. 1-3. ISSN 0166-6851. E-ISSN 1872-9428
Grant ostatní: Slovak Research and Development Agency(SK) APVV-15-0004; Slovak Grant Agency VEGA(SK) 2/0134/17; Centre of Excellence for Parasitology(SK) 26220120022
Institucionální podpora: RVO:60077344
Klíčová slova: Short tandem repeats * Population genetics * Microsatellites * Broad Fish tapeworm * Diphyllobothriidea * Microsatellites
Obor OECD: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Impakt faktor: 2.158, rok: 2018

The broad fish tapeworm Dibothriocephalus lams is a causative agent of human food-borne disease called diphyllobothriosis. Medical importance, scattered geographical distribution and unknown origin of D. latus in Europe and North America make this species to be an interesting model for population genetics. Microsatellite markers were originally designed by library screening using NGS approach and validated as tools for future studies on population genetics of D. latus. Out of 122 candidates selected after NGS analysis, 110 yielded PCR products of the expected size, and in 78 of them, a declared repetitive motif was confirmed by Sanger sequencing. After the fragment analysis, six loci were proved to be polymorphic and tested for observed (Ho) and expected (He) heterozygosity, and deviations from Hardy-Weinberg equilibrium (HWE). They promise future application in studies on genetic interrelationships, origin and migratory routes of this medically important emerging tapeworm.
Trvalý link: http://hdl.handle.net/11104/0291204