Electrospun vascular grafts fabricated from poly(L-lactide-co-ε-caprolactone) used as a bypass for the rabbit carotid artery

0496965 - ÚEM 2019 RIV GB eng J - Článek v odborném periodiku
Horáková, J. - Mikeš, P. - Lukáš, P. - Šaman, A. - Jenčová, V. - Klapstová, A. - Švarcová, T. - Ackermann, M. - Novotný, V. - Kalab, M. - Lonský, V. - Bartoš, M. - Rampichová, Michala - Litvinec, Andrej - Kubíková, T. - Tomášek, P. - Tonar, Z.
Electrospun vascular grafts fabricated from poly(L-lactide-co-ε-caprolactone) used as a bypass for the rabbit carotid artery.
Biomedical Materials. Roč. 13, č. 6 (2018), č. článku 065009. ISSN 1748-6041. E-ISSN 1748-605X
Institucionální podpora: RVO:68378041
Klíčová slova: vascular graft * electrospinning * copolymer poly-L-lactide-co-epsilon-caprolactone
Obor OECD: Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
Impakt faktor: 3.440, rok: 2018

The study involved the electrospinning of the copolymer poly L-lactide-co-epsilon-caprolactone PLCL into tubular grafts. The subsequent material characterization, including micro-computed tomography analysis, revealed a level of porosity of around 70%, with pore sizes of 9.34 +/- 0.19 mu m and fiber diameters of 5.58 +/- 0.10 mu m. Unlike fibrous polycaprolactone, the electrospun PLCL copolymer promoted fibroblast and endothelial cell adhesion and proliferation in vitro. Moreover, the regeneration of the vessel wall was detected following implantation and, after six months, the endothelialization of the lumen and the infiltration of arranged smooth muscle cells producing collagen was observed. However, the degradation rate was found to be accelerated in the rabbit animal model. The study was conducted under conditions that reflected the clinical requirements-the prostheses were sutured in the end-to-side fashion and the long-term end point of prosthesis healing was assessed. The regeneration of the vessel wall in terms of endothelialization, smooth cell infiltration and the presence of collagen fibers was observed after six months in vivo. A part of the grafts failed due to the rapid degradation rate of the PLCL copolymer.
Trvalý link: http://hdl.handle.net/11104/0294005