Osteoarthritic process modifies expression response to NiTi alloy presence

0496634 - ÚFM 2019 RIV NL eng J - Článek v odborném periodiku
Válková, L. - Ševčíková, J. - Goldbergová, M. P. - Weiser, Adam - Dlouhý, Antonín
Osteoarthritic process modifies expression response to NiTi alloy presence.
Journal of Materials Science-Materials in Medicine. Roč. 29, č. 9 (2018), č. článku 146. ISSN 0957-4530. E-ISSN 1573-4838
Grant CEP: GA ČR(CZ) GA15-16336S
Institucionální podpora: RVO:68081723
Klíčová slova: human articular chondrocytes * implant material * biocompatibility
Obor OECD: Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management
Impakt faktor: 2.467, rok: 2018

Nickel-titanium alloy (nitinol, NiTi) is a biomaterial with unique thermal shape memory, superelasticity and high damping properties. Therefore NiTi has been used in medical applications. In this in vitro study, the effect of NiTi alloy (with two surface modifications – helium and hydrogen) on gene expression profile of selected interleukins (IL-1β, IL-6 and IL-8) and matrix metalloproteinases (MMP-1 and MMP-2) in human physiological osteoblasts and human osteoarthritic osteoblasts was examined to respond to a question of the different behavior of bone tissue in the implantation of metallic materials in the presence of cells affected by the osteoarthritic process. The cells were cultivated in contact with NiTi and with or without LPS (bacterial lipolysaccharide). Changes in expression of target genes were calculated by 2-ΔΔCt method. An increased gene expression of IL-1β in osteoarthritic osteoblasts, with even higher expression in cells collected directly from the metal surface was observed. In case of physiological osteoblasts, the change in expression was detected after LPS treatment in cells surrounding the disc. Higher expression levels of IL-8 were observed in osteoarthritic osteoblasts after NiTi treatment in contact with alloy, and in physiological osteoblasts without relation to location in combination of NiTi and LPS. IL-6 was slightly increased in physiological osteoblastes after application of LPS. MMP-1 expression level was obviously significantly higher in osteoarthritic osteoblasts with differences regarding the metal surface and location. MMP-2 expression was decreased in both cell lines after LPS treatment. In conclusion, results of present study show that the NiTi alloy and the treatment by LPS, especially repeated doses of LPS, change the gene expression of selected ILs and MMPs in human osteoblast cell cultures. Some of the changes were depicted solely to osteoarthritic osteoblasts.
Trvalý link: http://hdl.handle.net/11104/0289411