0496525 - ÚMCH 2020 RIV NL eng J - Článek v odborném periodiku
Patsula, Vitalii - Moskvin, Maksym - Siow, W. X. - Konefal, Rafal - Ma, Y.-H. - Horák, Daniel
Antioxidant polymer-modified maghemite nanoparticles.
Journal of Magnetism and Magnetic Materials. Roč. 473, 1 March (2019), s. 517-526. ISSN 0304-8853. E-ISSN 1873-4766
Grant CEP: GA ČR(CZ) GC16-01128J
Institucionální podpora: RVO:61389013
Klíčová slova: magnetic * nanoparticles * antioxidant
Obor OECD: Polymer science
Impakt faktor: 2.717, rok: 2019
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S0304885318317438?via%3Dihub

Natural antioxidants, such as epigallocatechin-3-gallate and related phenolic compounds from tea, enhance particle cell-interactions and cellular uptake. In this study, surface of superparamagnetic iron oxide nanoparticles prepared by co-precipitation of Fe chlorides was modified with silica, polyethylenimine, poly(ethylene glycol), and poly(l-lysine) to protect the iron oxide core from redox-reactions with phenols, enhance uptake by the cells, prevent the particle aggregation, or enable conjugation with several phenol-based antioxidants. To reveal the relation between the particle uptake and chemical structure of the phenolic antioxidants, five of them were selected, namely phenol, phloroglucinol, chlorogenic, gallic, and tannic acid. After incubation of the phenol-modified nanoparticles with U87MG human glioma cells, intracellular levels of the reactive oxygen species were reduced in a similar manner, as measured by flow cytometry. Colorimetric iron assay revealed a comparable level of cell-associated particles, which was mostly independent on the applied external magnetic field. The results suggest that the poly(l-lysine)-based coating is responsible for the antioxidant effects of the particles. The phenol- and poly(l-lysine)-coatings enable effective colloidal stability in aqueous media and enhance cellular internalization of the nanoparticles.
Trvalý link: http://hdl.handle.net/11104/0289541