Plastidial Phosphoglucose Isomerase Is an Important Determinant of Seed Yield through Its Involvement in Gibberellin-Mediated Reproductive Development and Storage Reserve Biosynthesis in Arabidopsis

0495436 - ÚEB 2019 RIV US eng J - Článek v odborném periodiku
Bahaji, A. - Almagro, G. - Ezquer, I. - Gamez-Arcas, S. - Sanchez-Lopez, A.M. - Jose Munoz, F. - Barrio, R.J. - Sampedro, M.C. - De Diego, N. - Spíchal, L. - Doležal, Karel - Tarkowská, Danuše - Caporali, E. - Mendes, M.A. - Baroja-Fernandez, E. - Pozueta-Romero, J.
Plastidial Phosphoglucose Isomerase Is an Important Determinant of Seed Yield through Its Involvement in Gibberellin-Mediated Reproductive Development and Storage Reserve Biosynthesis in Arabidopsis.
Plant Cell. Roč. 30, č. 9 (2018), s. 2082-2098. ISSN 1040-4651. E-ISSN 1532-298X
Grant CEP: GA MŠMT(CZ) LO1204
Institucionální podpora: RVO:61389030
Klíčová slova: fatty-acid synthesis * microarray analysis * starch synthesis * metabolism * cytokinin * thaliana * pathway * genes * mutants * embryos
Obor OECD: Plant sciences, botany
Impakt faktor: 8.631, rok: 2018

The plastid-localized phosphoglucose isomerase isoform PGI1 is an important determinant of growth in Arabidopsis thaliana, likely due to its involvement in the biosynthesis of plastidial isoprenoid-derived hormones. Here, we investigated whether PGI1 also influences seed yields. PGI1 is strongly expressed in maturing seed embryos and vascular tissues. PGI1-null pgi1-2 plants had similar to 60% lower seed yields than wild-type plants, with reduced numbers of inflorescences and thus fewer siliques and seeds per plant. These traits were associated with low bioactive gibberellin (GA) contents. Accordingly, wild-type phenotypes were restored by exogenous GA application. pgi1-2 seeds were lighter and accumulated similar to 50% less fatty acids (FAs) and similar to 35% less protein than wild-type seeds. Seeds of cytokinin-deficient plants overexpressing CYTOKININ OXIDASE/DEHYDROGENASE1 (35S: AtCKX1) and GA-deficient ga20ox1 ga20ox2 mutants did not accumulate low levels of FAs, and exogenous application of the cytokinin 6-benzylaminopurine and GAs did not rescue the reduced weight and FA content of pgi1-2 seeds. Seeds from reciprocal crosses between pgi1-2 and wild-type plants accumulated wild-type levels of FAs and proteins. Therefore, PGI1 is an important determinant of Arabidopsis seed yield due to its involvement in two processes: GA-mediated reproductive development and the metabolic conversion of plastidial glucose-6-phosphate to storage reserves in the embryo.
Trvalý link: http://hdl.handle.net/11104/0288420