Počet záznamů: 1
Molecular and Cytogenetic Study of East African Highland Banana
- 1.0495318 - ÚEB 2019 RIV CH eng J - Článek v odborném periodiku
Němečková, Alžběta - Christelová, Pavla - Čížková, Jana - Nyine, Moses - Van den Houwe, I. - Svačina, Radim - Uwimana, B. - Swennen, R. - Doležel, Jaroslav - Hřibová, Eva
Molecular and Cytogenetic Study of East African Highland Banana.
Frontiers in Plant Science. Roč. 9, OCT 4 (2018), č. článku 1371. ISSN 1664-462X. E-ISSN 1664-462X
Grant CEP: GA MŠMT(CZ) LG12021; GA MŠMT(CZ) LO1204
Institucionální podpora: RVO:61389030
Klíčová slova: genetic diversity * diploid bananas * genome size * musa spp. * sequence alignment * provides insights * ribosomal dna * markers * domestication * chloroplast * East African highland bananas * fluorescence in situ hybridization (FISH) * ITS phylogeny * Musa * rRNA genes * simple sequence repeats genotyping
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 4.106, rok: 2018
East African highland bananas (EAHBs) are staple food crop in Uganda, Tanzania, Burundi, and other countries in the African Great Lakes region. Even though several morphologically different types exist, all EAHBs are triploid and display minimal genetic variation. To provide more insights into the genetic variation within EAHBs, genotyping using simple sequence repeat (SSR) markers, molecular analysis of ITS1-5.8S-ITS2 region of ribosomal DNA locus, and the analysis of chromosomal distribution of ribosomal DNA sequences were done. A total of 38 triploid EAHB accessions available in the Musa germplasm collection (International Transit Centre, Leuven, Belgium) were characterized. Six diploid accessions of Musa acuminata ssp. zebrina, ssp. banksii, and ssp. malaccensis representing putative parents of EAHBs were included in the study. Flow cytometric estimation of 2C nuclear DNA content revealed small differences (max similar to 6.5%) in genome size among the EAHB clones. While no differences in the number of 45S and 5S rDNA loci were found, genotyping using 19 SSR markers resulted in grouping the EAHB accessions into four clusters. The DNA sequence analysis of the internal transcribed spacer region indicated a relation of EAHB clones with M. acuminata and, surprisingly, also with M. schizocarpa. The results suggest that EAHB cultivars originated from a single hybrid clone with M. acuminata ssp. zebrina and ssp. banksii being its most probable parents. However, M. schizocarpa seems to have contributed to the formation of this group of banana.
Trvalý link: http://hdl.handle.net/11104/0288312
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Počet záznamů: 1