Počet záznamů: 1  

Aryl Hydrocarbon Receptor-Dependent Metabolism Plays a Significant Role in Estrogen-Like Effects of Polycyclic Aromatic Hydrocarbons on Cell Proliferation

  1. 1.
    0494990 - BFÚ 2019 RIV US eng J - Článek v odborném periodiku
    Hýžďalová, Martina - Pivnička, Jakub - Zapletal, Ondřej - Vazquez-Gomez, Gerardo - Matthews, J. - Neča, J. - Pěnčíková, K. - Machala, M. - Vondráček, Jan
    Aryl Hydrocarbon Receptor-Dependent Metabolism Plays a Significant Role in Estrogen-Like Effects of Polycyclic Aromatic Hydrocarbons on Cell Proliferation.
    Toxicological Sciences. Roč. 165, č. 2 (2018), s. 447-461. ISSN 1096-6080. E-ISSN 1096-0929
    Grant CEP: GA ČR(CZ) GA16-17085S
    Institucionální podpora: RVO:68081707
    Klíčová slova: breast-cancer cells * reporter gene assay * carcinoma mcf-7 cells * differential action * beta-isoform * dna-damage * cross-talk * er-beta * alpha * transcription
    Obor OECD: Biochemistry and molecular biology
    Impakt faktor: 3.564, rok: 2018

    Polycyclic aromatic hydrocarbons (PAHs) are widespread environmental contaminants that interact in a complex manner with both the aryl hydrocarbon receptor (AhR) and estrogen receptors (ER). Their potential endocrine-disrupting activities may depend on both inhibitory AhR-ER cross-talk and on AhR-dependent metabolic production of estrogenic PAH metabolites. Here, we analyzed the impact of AhR on estrogen-like effects of PAHs, such as benzotalpyrene (BaP), in particular, on control of cell cycle progression/cell proliferation. Using AhR knockout variant of estrogen-sensitive human breast cancer MCF-7 cells (MCF-7 AhR(KO) cells), we observed that the AhR-dependent control of cytochrome P450 family 1 (CYP1) expression played a major role in formation of estrogenic BaP metabolites, most notably 3-OH-BaP, which contributed to the ER-dependent induction of cell cycle progression/cell proliferation. Both BaP metabolism and the BaP-induced S-phase transition/cell proliferation were inhibited in MCF-7 AhR(KO) cells, whereas these cells remained sensitive towards both endogenous estrogen 17 beta-estradiol or hydroxylated BaP metabolites. BaP was found to increase the activity of ER-dependent luciferase reporter gene in wild-type MCF-7 cells, however, unlike its hydroxylated metabolite, BaP failed to stimulate luciferase activity in MCF-7 AhR(KO) cells. Similarly, estrogen-like effects of other known estrogenic PAHs, such as benzfajanthracene or 3-methylcholanthrene, were diminished in MCF-7 AhR(KO) cells. Ectopic expression of human CYP1A1 and CYP1B1 enzymes partly restored both BaP metabolism and its effects on cell proliferation. Taken together, our data suggest that the AhR-dependent metabolism of PAHs contributes significantly to the impact of PAHs on cell proliferation in estrogen-sensitive cells.
    Trvalý link: http://hdl.handle.net/11104/0288039

     
     
Počet záznamů: 1  

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