Expression of TIM-3 on Plasmacytoid Dendritic Cells as a Predictive Biomarker of Decline in HIV-1 RNA Level during ART

0494673 - ÚMG 2019 RIV CH eng J - Článek v odborném periodiku
Font-Haro, Albert - Janovec, Vaclav - Hofman, T. - Machala, L. - Jilich, D. - Mělková, Z. - Weber, J. - Trejbalová, Kateřina - Hirsch, Ivan
Expression of TIM-3 on Plasmacytoid Dendritic Cells as a Predictive Biomarker of Decline in HIV-1 RNA Level during ART.
Viruses. Roč. 10, č. 4 (2018), č. článku 154. E-ISSN 1999-4915
Grant CEP: GA ČR GA14-32547S; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) LQ1604
Institucionální podpora: RVO:68378050
Klíčová slova: hiv-1 * antiretroviral therapy (ART) * innate and adaptive immune responses * plasmacytoid dendritic cells (pDCs) * pDC dysfunction * T cell Ig and mucin-domain containing molecule 3 (TIM-3) * bdca-2 * Toll-like receptors 7 and 9 (TLR7/9)
Obor OECD: Virology
Impakt faktor: 3.811, rok: 2018

Depletion and functional impairment of circulating plasmacytoid dendritic cells (pDCs) are characteristic attributes of HIV-1-infection. The mechanism of dysfunction of pDCs is unclear. Here, we studied the development of phenotype of pDCs in a cohort of HIV-1-infected individuals monitored before the initiation and during a 9-month follow up with antiretroviral therapy (ART). Using polychromatic flow cytometry, we detected significantly higher pDC-surface expression of the HIV-1 receptor CD4, regulatory receptor BDCA-2, Fcy receptor CD32, pDC dysfunction marker TIM-3, and the marker of killer pDC, TRAIL, in treatment-naive HIV-1-infected individuals before initiation of ART when compared to healthy donors. After 9 months of ART, all of these markers approached but did not reach the expression levels observed in healthy donors. We found that the rate of decline in HIV-1 RNA level over the first 3 months of ART negatively correlated with the expression of TITM-3 on pDCs. We conclude that immunogenic phenotype of pDCs is not significantly restored after sustained suppression of HIV-1 RNA level in ART-treated patients and that the level of the TIM-3 expressed on pDCs in treatment naive patients could be a predictive marker of the rate of decline in the HIV-1 RNA level during ART.
Trvalý link: http://hdl.handle.net/11104/0287774