Locking in on large volume light-sheet microscopy

0494379 - ÚPT 2019 CZ eng C - Konferenční příspěvek (zahraniční konf.)
Vettenburg, T. - Dalgarno, H.I.C. - Nylk, J. - Coll-Lladó, C. - Ferrier, D.E.K. - Čižmár, Tomáš - Gunn-Moore, F.J. - Dholakia, K. - Corral, A. - Rodriguez-Pulido, A. - Flors, C. - Ripoll, J.
Locking in on large volume light-sheet microscopy.
Recent Trends in Charged Particle Optics and Surface Physics Instrumentation. Proceedings of the 16th International Seminar. Brno: Institute of Scientific Instruments The Czech Academy of Sciences, 2018, s. 84-85. ISBN 978-80-87441-23-7.
[Recent Trends in Charged Particle Optics and Surface Physics Instrumentation. Skalský dvůr (CZ), 04.06.2018-08.06.2018]
Institucionální podpora: RVO:68081731
Klíčová slova: cellular imaging * fluorescence imaging * light-sheet microscopy
Obor OECD: Optics (including laser optics and quantum optics)

Fluorescence light-sheet microscopy is increasingly adopted by developmental biologists to study how cells divide and differentiate to form organs and even entire organisms. The lightsheet microscope differs from a conventional microscope in that the specimen is illuminated by a plane of light orthogonal to the detection axis, thus keeping the out-of-focus areas dark while minimizing any potentially detrimental exposure of the sample. The light-sheet microscope has been found to be the ideal instrument for long-term and non-invasive studies of intact, and therefore three-dimensional, fluorescent samples.
Trvalý link: http://hdl.handle.net/11104/0287640