Photon-upconverting nanoparticles as a novel background-free label in immunoassays

0494132 - ÚIACH 2019 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Farka, Z. - Hlaváček, Antonín - Mickert, M. J. - Skládal, P. - Gorris, H. H.
Photon-upconverting nanoparticles as a novel background-free label in immunoassays.
NANOCON 2017: Conference Proceedings. Ostrava: Tanger Ltd., 2018, s. 455-460. ISBN 978-80-87294-81-9.
[NANOCON 2017. International Conference on Nanomaterials - Research and Application /9./. Brno (CZ), 18.10.2017-20.10.2017]
Grant CEP: GA ČR(CZ) GBP206/12/G014
Institucionální podpora: RVO:68081715
Klíčová slova: photon-upconversion nanoparticle * immunoassay * bioconjugation * antibody * diclofenac
Obor OECD: Analytical chemistry

Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without optical background interference. Further advantages of UCNPs include good photostability, large anti-Stokes shifts, and multiple narrow emission bands that can be used for multiplexed detection. We have developed a competitive upconversion-linked immunosorbent assay (ULISA) for detection of the pharmaceutical diclofenac (DCF) in surface waters. Silica-coated UCNPs (50 nm in diameter) with carboxyl groups on the surface were synthesized and conjugated with the secondary anti-IgG antibody. The structure and monodispersity of the nanoconjugates was studied by TEM and agarose gel electrophoresis. Using a highly affine anti-DCF primary antibody, the optimized ULISA provided a detection limit of 50 pg·mL−1.
Trvalý link: http://hdl.handle.net/11104/0287392