Preparative DNA concentration by Epitachophoresis

0493911 - ÚIACH 2019 CH eng A - Abstrakt
Foret, František - Datinská, Vladimíra - Voráčová, Ivona - Gheibi, P. - Berka, J. - Astier, Y.
Preparative DNA concentration by Epitachophoresis.
Public Health Genomics. Roč. 21 (2018), s. 18-18. ISSN 1662-4246. E-ISSN 1662-8063.
[2018 Golden Helix Summer. 26.09.2018-30.09.2018, Syros Island]
Grant CEP: GA MŠMT(CZ) 8F17003
Institucionální podpora: RVO:68081715
Klíčová slova: epitachophoresis * DNA Concentration * capillary ITP
Obor OECD: Analytical chemistry

In conclusion, we have developed two simple, preparative
methods for DNA concentration and purification. The first method,
based on a capillary ITP, can process sample volumes up to 150
microliters. The second method, based on the flat channel design,
allows processing of 15 ml sample volumes. This is by far the largest
described focusing capacity. In the discontinuous electrolyte
system, the sample enrichment factor is limited only by the system
geometry and even higher loading capacities are possible if needed.
Trvalý link: http://hdl.handle.net/11104/0287180