Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

0493723 - ÚIACH 2019 RIV US eng J - Článek v odborném periodiku
Šťastná, Miroslava - Thomas, A. - Germano, J. - Pourpirali, S. - Van Eyk, J.E. - Gottlieb, R. A.
Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion.
Jove-Journal of Visualized Experiments. Roč. 2018, č. 138 (2018), č. článku e58079. ISSN 1940-087X
Institucionální podpora: RVO:68081715
Klíčová slova: dynamic proteomics * ischemia and reperfusion * protein translation
Obor OECD: Analytical chemistry
Impakt faktor: 1.108, rok: 2018
https://www.jove.com/video/58079

Studies in dynamic changes in protein translation require specialized methods. In this paper, we examine changes in newly-synthesized proteins in response to ischemia and reperfusion using the isolated perfused mouse heart coupled with polysome profiling. We present the methods that were used to analyze the distribution of proteins, mRNAs, and miRNAs in the fractions after sucrose gradient centrifugation. Specifically, in the high-efficiency fractions (numerous ribosomes attached to mRNA), low-efficiency fractions (fewer ribosomes attached), and the non-translating fractions.
Trvalý link: http://hdl.handle.net/11104/0287046