6,7-dimethoxy-coumarin as a probe of hydration dynamics in biologically relevant systems

0493474 - ÚFCH JH 2019 RIV GB eng J - Článek v odborném periodiku
Ghose, Avisek - Amaro, Mariana - Kovaříček, Petr - Hof, Martin - Sýkora, Jan
6,7-dimethoxy-coumarin as a probe of hydration dynamics in biologically relevant systems.
Methods and Applications in Fluorescence. Roč. 6, č. 2 (2018), č. článku 025005. ISSN 2050-6120. E-ISSN 2050-6120
Grant CEP: GA ČR(CZ) GA16-06096S
Institucionální podpora: RVO:61388955
Klíčová slova: proton-transfer reactions * aot reverse micelles * solvation dynamics * photophysical properties * stokes shift * tunnel mouth * live cell * fluorescence
Obor OECD: Physical chemistry
Impakt faktor: 2.940, rok: 2018

Coumarin derivatives are well known fluorescence reporters for investigating biological systems due to their strong micro-environment sensitivity. Despite having wide range of environment sensitive fluorescence probes, the potential of 6,7-dimethoxy-coumarin has not been studied extensively so far. With a perspective of its use in protein studies, namely using the unnatural amino acid technology or as a substrate for hydrolase enzymes, we study acetyloxymethyl-6,7-dimethoxycoumarin (Ac-DMC). We investigate the photophysics and hydration dynamics of this dye in aerosol-OT (AOT) reverse micelles at various water contents using the time dependent fluorescence shift (TDFS) method. The TDFS response in AOT reverse micelles from water/surfactant ratio of 0 to 20 confirms its sensitivity towards the hydration and mobility of its microenvironment. Moreover, we show that the fluorophore can be efficiently quenched by halide ions. Hence, we conclude that the 6,7-dimethoxy-methylcoumarin fluorophore is useful for studying hydration parameters in biologically relevant systems.
Trvalý link: http://hdl.handle.net/11104/0286837