The Effect of Human Mesenchymal Stem Cells Derived from Wharton's Jelly in Spinal Cord Injury Treatment Is Dose-Dependent and Can Be Facilitated by Repeated Application

0492882 - ÚEM 2019 RIV CH eng J - Článek v odborném periodiku
Krůpa, Petr - Vacková, Irena - Růžička, Jiří - Závišková, Kristýna - Dubišová, Jana - Kočí, Zuzana - Turnovcová, Karolína - Machová-Urdzíková, Lucia - Kubinová, Šárka - Řehák, S. - Jendelová, Pavla
The Effect of Human Mesenchymal Stem Cells Derived from Wharton's Jelly in Spinal Cord Injury Treatment Is Dose-Dependent and Can Be Facilitated by Repeated Application.
International Journal of Molecular Sciences. Roč. 19, č. 5 (2018), č. článku 1503. E-ISSN 1422-0067
Grant CEP: GA MŠMT(CZ) EF15_003/0000419; GA ČR(CZ) GBP304/12/G069; GA ČR(CZ) GA17-03765S; GA MŠMT(CZ) LM2015064; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) LQ1604
Institucionální podpora: RVO:68378041
Klíčová slova: spinal cord injury * human mesenchymal stem cells * Wharton's jelly * inflammatory response
Obor OECD: Neurosciences (including psychophysiology
Impakt faktor: 4.183, rok: 2018

Human mesenchymal stem cells derived from Wharton's jelly (WJ-MSCs) were used for the treatment of the ischemic-compression model of spinal cord injury in rats. To assess the effectivity of the treatment, different dosages (0.5 or 1.5 million cells) and repeated applications were compared. Cells or saline were applied intrathecally by lumbar puncture for one week only, or in three consecutive weeks after injury. Rats were assessed for locomotor skills (BBB, rotarod, flat beam) for 9 weeks. Spinal cord tissue was morphometrically analyzed for axonal sprouting, sparing of gray and white matter and astrogliosis. Endogenous gene expression (Gfap, Casp3, Irf5, Cd86, Mrc1, Cd163) was studied with quantitative Real-time polymerase chain reaction (qRT PCR). Significant recovery of functional outcome was observed in all of the treated groups except for the single application of the lowest number of cells. Histochemical analyses revealed a gradually increasing effect of grafted cells, resulting in a significant increase in the number of GAP43+ fibers, a higher amount of spared gray matter and reduced astrogliosis. mRNA expression of macrophage markers and apoptosis was downregulated after the repeated application of 1.5 million cells. We conclude that the effect of hWJ-MSCs on spinal cord regeneration is dose-dependent and potentiated by repeated application.
Trvalý link: http://hdl.handle.net/11104/0286490