Dynamics of tissue ingrowth in SIKVAV-modified highly superporous PHEMA scaffolds with oriented pores after bridging a spinal cord transection

0492877 - ÚEM 2019 RIV GB eng J - Článek v odborném periodiku
Hejčl, Aleš - Růžička, Jiří - Proks, Vladimír - Macková, Hana - Kubinová, Šárka - Tukmachev, D. - Cihlář, J. - Horák, Daniel - Jendelová, Pavla
Dynamics of tissue ingrowth in SIKVAV-modified highly superporous PHEMA scaffolds with oriented pores after bridging a spinal cord transection.
Journal of Materials Science-Materials in Medicine. Roč. 29, č. 7 (2018), č. článku 89. ISSN 0957-4530. E-ISSN 1573-4838
Grant CEP: GA ČR(CZ) GA14-14961S; GA ČR(CZ) GA17-11140S; GA MŠMT(CZ) LO1309; GA MŠMT(CZ) EF15_003/0000419
Institucionální podpora: RVO:68378041 ; RVO:61389013
Klíčová slova: mesenchymal STEM-CELLS * sensory axon regenerationsensory axon regeneration * endothelial growth-factor * functional recovery
Obor OECD: Neurosciences (including psychophysiology; Polymer science (UMCH-V)
Impakt faktor: 2.467, rok: 2018

While many types of biomaterials have been evaluated in experimental spinal cord injury (SCI) research, little is known about the time-related dynamics of the tissue infiltration of these scaffolds. We analyzed the ingrowth of connective tissue, axons and blood vessels inside the superporous poly (2-hydroxyethyl methacrylate) hydrogel with oriented pores. The hydrogels, either plain or seeded with mesenchymal stem cells (MSCs), were implanted in spinal cord transection at the level of Th8. The animals were sacrificed at days 2, 7, 14, 28, 49 and 6 months after SCI and histologically evaluated. We found that within the first week, the hydrogels were already infiltrated with connective tissue and blood vessels, which remained stable for the next 6 weeks. Axons slowly and gradually infiltrated the hydrogel within the first month, after which the numbers became stable. Six months after SCI we observed rare axons crossing the hydrogel bridge and infiltrating the caudal stump. There was no difference in the tissue infiltration between the plain hydrogels and those seeded with MSCs. We conclude that while connective tissue and blood vessels quickly infiltrate the scaffold within the first week, axons show a rather gradual infiltration over the first month, and this is not facilitated by the presence of MSCs inside the hydrogel pores. Further research which is focused on the permissive micro-environment of the hydrogel scaffold is needed, to promote continuous and long-lasting tissue regeneration across the spinal cord lesion.
Trvalý link: http://hdl.handle.net/11104/0286333