Herbarium tale: the utility of dry specimens for DNA barcoding Juncaceae

0492148 - BÚ 2019 RIV AT eng J - Článek v odborném periodiku
Do, D. - Záveská Drábková, Lenka
Herbarium tale: the utility of dry specimens for DNA barcoding Juncaceae.
Plant Systematics and Evolution. Roč. 304, č. 2 (2018), s. 281-294. ISSN 0378-2697. E-ISSN 1615-6110
Grant CEP: GA ČR(CZ) GA16-14649S; GA ČR(CZ) GAP506/11/0774
Institucionální podpora: RVO:67985939
Klíčová slova: DNA barcoding * dry plants * herbarium specimens * Juncaceae
Obor OECD: Plant sciences, botany
Impakt faktor: 1.585, rok: 2018

The herbarium specimens amplified well and may thus be successfully applied for both phylogenetic analyses and barcoding for the Juncaceae family. Amplifying DNA was more difficult from dried herbarium specimens than fresh samples but could be successful in most cases when appropriate internal primers were designed or methods were optimised. Using the set of universal primers recommended by the Consortium for the Barcode of Life and designing specific primers for a particular group of interest were both useful. Specimen age and amplicon length had limited detrimental effects on amplification success for most of the Juncaceae loci tested.
Trvalý link: http://hdl.handle.net/11104/0285733