Determination of Storage (Starch/Glycogen) and Total Saccharides Content in Algae and Cyanobacteria by a Phenol-Sulfuric Acid Method

0492137 - ÚVGZ 2019 RIV US eng J - Článek v odborném periodiku
Zavřel, Tomáš - Očenášová, Petra - Sinětova, M. A. - Červený, Jan
Determination of Storage (Starch/Glycogen) and Total Saccharides Content in Algae and Cyanobacteria by a Phenol-Sulfuric Acid Method.
Bio-protocol. Roč. 8, č. 15 (2018), č. článku e2966. ISSN 2331-8325
Grant CEP: GA MŠMT(CZ) LO1415; GA ČR(CZ) GA15-17367S; GA MŠMT(CZ) LM2015055
Institucionální podpora: RVO:86652079
Klíčová slova: Sugars * Carbohydrates * Polysaccharides * Colorimetry * Spectrophotometry * Chlorella * Haematococcus
Obor OECD: Biochemical research methods
Způsob publikování: Omezený přístup
https://bio-protocol.org/e2966

This is a protocol for quantitative determination of storage and total carbohydrates in algae and cyanobacteria. The protocol is simple, fast and sensitive and it requires only few standard chemicals. Great advantage of this protocol is that both storage and total saccharides can be determined in the cellular pellets that were already used for chlorophyll and carotenoids quantification. Since it is recommended to perform the pigments measurement in triplicates, each pigment analysis can generate samples for both total saccharide and glycogen/starch content quantification. The protocol was applied for quantification of both storage and total carbohydrates in cyanobacteria Synechocystis sp. PCC 6803, Cyanothece sp. ATCC 51142 and Cyanobacterium sp. IPPAS B-1200. It was also applied for estimation of storage polysaccharides in Galdieria (IPPAS P-500, IPPAS P-507,IPPAS P-508, IPPAS P-513), Cyanidium caldarium IPPAS P-510, in green algae Chlorella sp. IPPAS C- 1 and C-1210, Parachlorella kessleri IPPAS C-9, Nannochloris sp. C-1509, Coelastrella sp. IPPAS H-626, Haematococcus sp. IPPAS H-629 and H-239, and in Eustigmatos sp. IPPAS H-242 and IPPAS C-70.
Trvalý link: http://hdl.handle.net/11104/0285691