Turning Off Transcription with Bacterial RNA Polymerase through CuAAC Click Reactions of DNA Containing 5-Ethynyluracil

0491770 - ÚOCHB 2019 RIV DE eng J - Článek v odborném periodiku
Slavíčková, Michaela - Janoušková, Martina - Šimonová, Anna - Cahová, Hana - Kambová, Milada - Šanderová, Hana - Krásný, Libor - Hocek, Michal
Turning Off Transcription with Bacterial RNA Polymerase through CuAAC Click Reactions of DNA Containing 5-Ethynyluracil.
Chemistry - A European Journal. Roč. 24, č. 33 (2018), s. 8311-8314. ISSN 0947-6539. E-ISSN 1521-3765
Grant CEP: GA ČR(CZ) GA17-03419S
Grant ostatní: AV ČR(CZ) AP1501
Program: Akademická prémie - Praemium Academiae
Institucionální podpora: RVO:61388963 ; RVO:61388971
Klíčová slova: DNA * epigenetics * nucleotides * RNA polymerase * transcription
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 5.160, rok: 2018

Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction in the major groove of DNA containing 5-ethynyluracil (U-E) with azides was used for turning off sequence-specific protein-DNA interactions. The concept was first demonstrated on switching off cleavage of short modified DNA by restriction endonuclease BamHI-HF. Finally, DNA template containing U-E was used for in vitro transcription with E.coli RNA polymerase and the transcription was turned off by CuAAC with 3-azidopropane-1,2-diol or 3-azido-7-hydroxycoumarin.
Trvalý link: http://hdl.handle.net/11104/0285408