Silica capillary monolithic columns - synthesis, immobilization and chromatographic properties of novel phosphonium ionic liquid stationary phase

0491041 - ÚIACH 2019 SK eng A - Abstrakt
Planeta, Josef - Moravcová, Dana - King, A. - Wiedmer, S. K. - Karásek, Pavel - Roth, Michal
Silica capillary monolithic columns - synthesis, immobilization and chromatographic properties of novel phosphonium ionic liquid stationary phase.
Book of abstracts. 24th International Symposium on Separation Sciences (ISSS 2018). Bratislava: Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, 2018 - (Hutta, M.; Bodor, R.; Halko, R.). s. 88-88. ISBN 978-80-971179-8-6.
[International Symposium on Separation Sciences /24./ - ISSS 2018, International Conference Analytical Methods and Human Health /21./ - AMHH 2018. 17.06.2018-20.06.2018, Jasná]
Grant CEP: GA ČR(CZ) GA16-03749S; GA MV VI20172020069
Institucionální podpora: RVO:68081715
Klíčová slova: silica capillary monolithic columns * ionic liquids stationary phase * HPLC separation
Obor OECD: Analytical chemistry

Our work demonstrates the preparation of silica-monolithic capillary columns (100 μm i.d. x 150 mm)
by hydrolysis of tetramethoxysilane in the presence of polyethylene glycol and urea under acidic conditions
at elevated temperature. Details of the column manufacturing process as well as SEM pictures are
presented. Prepared silica support was covered by novel phosphonium ionic liquid trioctyl (3/4-vinylbenzyl)
phosphonium chloride. Synthesis route of monomer and its NMR spectra are presented. Prepared
stationary phase was bonded by radical polymerization of IL with 3-(trimethoxysilyl) propyl methacrylate
to silica monolithic column. The synthetized column with phosphonium IL stationary phase presents multiple kinds of retention mechanisms – it works in HILIC chromatography, reversed-phase chromatography and in ion exchange
chromatography. Separation properties are demonstrated on various sample mixtures and evaluated.
Trvalý link: http://hdl.handle.net/11104/0285122