Morphology of a fibrin nanocoating influences dermal fibroblast behavior

0490959 - FGÚ 2019 RIV NZ eng J - Článek v odborném periodiku
Pajorová, Julia - Bačáková, Markéta - Musílková, Jana - Brož, Antonín - Hadraba, Daniel - Lopot, F. - Bačáková, Lucie
Morphology of a fibrin nanocoating influences dermal fibroblast behavior.
International Journal of Nanomedicine. Roč. 13, June (2018), s. 3367-3380. E-ISSN 1178-2013
Grant CEP: GA ČR(CZ) GA17-02448S; GA MŠMT(CZ) LM2015062
Institucionální podpora: RVO:67985823
Klíčová slova: nanofibers * fibrin * nanocoating * dermal fibroblasts * extracellular matrix synthesis * skin substitute * polylactic acid
Obor OECD: Nano-processes (applications on nano-scale)
Impakt faktor: 4.471, rok: 2018

Our study focuses on the fabrication of appropriate scaffolds for skin wound healing. This research brings valuable insights into the molecular mechanisms of adhesion, proliferation, and control of cell behavior through the extracellular matrix represented by synthetic biodegradable nanofibrous membranes coated by biomolecules.Nanofibrous polylactic acid (PLA) membranes were prepared by a needle-less electrospinning technology. These membranes were coated with fibrin according to two preparation protocols, and additionally they were coated with fibronectin in order to increase the cell affinity for colonizing the PLA membranes. The adhesion, growth, and extracellular matrix protein production of neonatal human dermal fibroblasts were evaluated on the nanofibrous membranes.Our results showed that fibrin-coated membranes improved the adhesion and proliferation of human dermal fibroblasts. The morphology of the fibrin nanocoating seems to be crucial for the adhesion of fibroblasts, and consequently for their phenotypic maturation. Fibrin either covered the individual fibers in the membrane (F1 nanocoating), or covered the individual fibers and also formed a fine homogeneous nanofibrous mesh on the surface of the membrane (F2 nanocoating), depending on the mode of fibrin preparation. The fibroblasts on the membranes with the F1 nanocoating remained in their typical spindle-like shape. However, the cells on the F2 nanocoating were spread mostly in a polygon-like shape, and their proliferation was significantly higher. Fibronectin formed an additional mesh attached to the surface of the fibrin mesh, and further enhanced the cell adhesion and growth. The relative gene expression and protein production of collagen I and fibronectin were higher on the F2 nanocoating than on the F1 nanocoating.A PLA membrane coated with a homogeneous fibrin mesh seems to be promising for the construction of temporary full-thickness skin tissue substitutes.
Trvalý link: http://hdl.handle.net/11104/0285061