Amperometric Determination of Catecholamines by Enzymatic Biosensors in Flow Systems

0490697 - ÚFCH JH 2019 RIV DE eng J - Článek v odborném periodiku
Josypčuk, Oksana - Barek, J. - Josypčuk, Bohdan
Amperometric Determination of Catecholamines by Enzymatic Biosensors in Flow Systems.
Electroanalysis. Roč. 30, č. 6 (2018), s. 1163-1171. ISSN 1040-0397. E-ISSN 1521-4109
Grant CEP: GA ČR(CZ) GA15-03139S; GA ČR GA17-03868S
Institucionální podpora: RVO:61388955
Klíčová slova: Amperometry * Catecholamines * Enzymatic biosensor * Flow analysis * Silver amalgam
Obor OECD: Physical chemistry
Impakt faktor: 2.691, rok: 2018

Four various biosensors based on flow enzymatic mini-reactors containing one of the two different mesoporous silica powders (SBA-15 or MCM-41) modified by one of the two enzymes (laccase or tyrosinase) were used for the determination of L-DOPA, dopamine, noradrenaline, and adrenaline. Dependences of peak currents on detection potentials, flow rates, injected volumes, reactor volumes, and analyte concentrations were studied and optimized. The silica powder of MCM-41 with covalently bonded laccase (Lac) was found to be the best filling for the detection of catecholamines. Relative current response of such biosensor was: 100 % for dopamine, 32.1 % for L-DOPA, 26.2 % for noradrenaline, and 0.71 % for adrenaline. Statistical evaluation of parallel determinations of studied analytes with this biosensor proved its good accuracy (RSD=1.82–5.18 %) and sensitivity (5.46–15.5 μmol L−1). Life time of the studied biosensors is several months. The practical applicability of Lac-MCM41 biosensor was successfully verified by the determination of dopamine and noradrenaline in medical solutions for infusions.
Trvalý link: http://hdl.handle.net/11104/0284872