Nanomaterials in Biomedical research

0490615 - ÚEM 2019 RIV CZ eng M - Část monografie knihy
Brzicová, Táňa - Líbalová, Helena - Vrbová, Kristýna - Sikorová, Jitka - Philimonenko, Vlada - Kléma, J. - Topinka, Jan - Rössner ml., Pavel
Whole-genome Expression Analysis in THP-1 Macrophage-like Cells Exposed to Diverse Nanomaterials.
Nanomaterials in Biomedical research. Praha 3: Proxima Tisk, s.r.o., 2017 - (Caraglia, M.), s. 9-14. ISBN 978-80-906655-5-2
Grant CEP: GA MŠMT(CZ) LO1508
Institucionální podpora: RVO:68378041 ; RVO:68378050
Klíčová slova: nanomaterials * toxicity * THP-1 macrophages * gene expression profiling
Obor OECD: Biochemistry and molecular biology

In the present study, we compared toxicity of four different types of NMs [NM-100 (TiO2, 110 nm), NM-110 (ZnO, 20 nm), NM-200 (SiO2, 150 nm) and NM-300K (Ag, 20 nm)] in THP-1 macrophage-like cells as a model cells of the first line of defense in the organism. Cells were incubated with non-cytotoxic concentrations (1-25 µg/ml) of NMs for 24 hours and microarray technology was used to analyze changes in whole-genome expression. NM-100 did not exert any significant effect, all other NMs triggered a pro-inflammatory response and induced expression of numerous chemokines and cytokines. NM-110 and NM-300K further modulated processes such as DNA damage response, oxidative stress as well as cell cycle progression and proteasome function.
Trvalý link: http://hdl.handle.net/11104/0285814