Visualizing Single-Cell Secretion Dynamics with Single-Protein Sensitivity

0490472 - ÚFE 2019 US eng J - Článek v odborném periodiku
McDonald, M.P. - Gemeinhardt, A. - König, K. - Piliarik, Marek - Schaffert, S. - Völkl, S. - Aigner, M. - Mackensen, A. - Sandoghdar, V.
Visualizing Single-Cell Secretion Dynamics with Single-Protein Sensitivity.
Nano Letters. Roč. 18, č. 1 (2018), s. 513-519. ISSN 1530-6984. E-ISSN 1530-6992
Institucionální podpora: RVO:67985882
Klíčová slova: cellular secretion * iSCAT * single-protein
Obor OECD: Optics (including laser optics and quantum optics)
Impakt faktor: 12.279, rok: 2018

ellular secretion of proteins into the extracellular environment is an essential mediator of critical biological mechanisms, including cell-to-cell communication, immunological response, targeted delivery, and differentiation. Here, we report a novel methodology that allows for the real-time detection and imaging of single unlabeled proteins that are secreted from individual living cells. This is accomplished via interferometric detection of scattered light (iSCAT) and is demonstrated with Laz388 cells, an Epstein Barr virus (EBV)-transformed B cell line. We find that single Laz388 cells actively secrete IgG antibodies at a rate of the order of 100 molecules per second. Intriguingly, we also find that other proteins and particles spanning ca. 100 kDa-1 MDa are secreted from the Laz388 cells in tandem with IgG antibody release, likely arising from EBV-related viral proteins. The technique is general and, as we show, can also be applied to studying the lysate of a single cell. Our results establish label-free iSCAT imaging as a powerful tool for studying the real-time exchange between cells and their immediate environment with single-protein sensitivity
Trvalý link: http://hdl.handle.net/11104/0284704