Počet záznamů: 1  

Electrochemical and AFM study of the interaction of recombinant human cathelicidin LL-37 with various supported bilayer lipid membranes

  1. 1.
    0490127 - ÚFCH JH 2019 RIV CH eng J - Článek v odborném periodiku
    Gál, M. - Sokolová, Romana - Naumowicz, M. - Híveš, J. - Krahulec, J.
    Electrochemical and AFM study of the interaction of recombinant human cathelicidin LL-37 with various supported bilayer lipid membranes.
    Journal of Electroanalytical Chemistry. Roč. 821, JUL 2018 (2018), s. 40-46. ISSN 1572-6657. E-ISSN 1873-2569
    Institucionální podpora: RVO:61388955
    Klíčová slova: cathelicidin * antibiotic resistance * LL-37
    Obor OECD: Analytical chemistry
    Impakt faktor: 3.218, rok: 2018

    The overuse of antibiotics is strongly associated with the development of the bacterial resistance which means that novel antimicrobial agents with different mode of action must be prepared. Antimicrobial peptides (AMP) can serve as almost an ideal candidate for the new generation of antimicrobial compounds. In this work the interactions of recombinantly prepared cathelicidin LL-37 towards various types and mixtures of supported bilayer lipid membranes (s-BLMs) are studied by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and atomic force microscopy (AFM). All of these methods have approved excellent efficiency of LL-37 towards membranes that are typical for prokaryotic organisms composed mainly of phosphatidylglycerol and lipid A. On the contrary, almost zero activity towards phosphatidylcholine membranes that are more frequently found in the structure of eukaryotic organisms was observed. A 'carpet model' where the peptides interact primarily with the lipid head groups, have been proved as a mode of LL-37 action. This finding is based on the results obtained by CV and EIS techniques and was revealed by the visualization of the electrode surface by AFM technique.
    Trvalý link: http://hdl.handle.net/11104/0284415

     
    Název souboruStaženoVelikostKomentářVerzePřístup
    0490127.pdf1631.6 KBVydavatelský postprintvyžádat
     
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.