Tuning phospholipid bilayer permeability by flavonoid apigenin: Electrochemical and atomic force microscopy study

0490126 - ÚFCH JH 2019 RIV CH eng J - Článek v odborném periodiku
Kocábová, Jana - Kolivoška, Viliam - Gál, M. - Sokolová, Romana
Tuning phospholipid bilayer permeability by flavonoid apigenin: Electrochemical and atomic force microscopy study.
Journal of Electroanalytical Chemistry. Roč. 821, JUL 2018 (2018), s. 67-72. ISSN 1572-6657. E-ISSN 1873-2569
Institucionální podpora: RVO:61388955
Klíčová slova: Apigenin * Atomic force microscopy and nanolithography * Cyclic voltammetry * Electrochemical impedance spectroscopy * Electron transfer kinetics * Supported lipid bilayer
Obor OECD: Analytical chemistry
Impakt faktor: 3.218, rok: 2018

The ability to tune the permeability of cell membranes might have significant implications in targeted drug delivery applications. In this work we employ electrochemical methods as well as nanolithography realized in the environment of in-situ atomic force microscopy (AFM) to demonstrate that bioactive compound apigenin is capable of altering the permeability of a model phospholipid dipalmitoylphosphatidylcholine bilayer supported by a gold surface. The electron transfer rate through the bilayer was investigated employing [Fe(CN) 6 ] 4− /[Fe(CN) 6 ] 3− redox couple as an electroactive probe. The value of the standard heterogeneous rate constant obtained in the absence of apigenin (k 0 = 6.60∙10 −5 cm·s −1 ) indicates that the electron transfer through the bilayer is rather sluggish confirming its compactness. The addition of apigenin to the system leads to a considerable increase of the electron transfer rate to k 0 = 5.74∙10 −3 cm·s −1 being virtually identical to the value obtained at the bare electrode/electrolyte interface (k 0 = 2.24∙10 −3 cm·s −1 ). This suggests that apigenin induces a significant rearrangement of the supported lipid bilayer increasing thus its permeability. The AFM nanolithography performed in-situ in the presence of apigenin confirmed that the supported lipid bilayer reorganizes itself to a monolayer of molecules laying parallel to the electrode surface.
Trvalý link: http://hdl.handle.net/11104/0284414