Alimentary tract transcriptome analysis of the tea geometrid, Ectropis oblique (Lepidoptera: Geometridae)

0490078 - BC 2019 RIV US eng J - Článek v odborném periodiku
Wang, J. - Lin, G. - Batool, K. - Zhang, S. - Chen, M. - Xu, J. - Wu, J. - Jin, L. - Gelbič, Ivan - Xu, L. - Zhang, L. - Guan, X.
Alimentary tract transcriptome analysis of the tea geometrid, Ectropis oblique (Lepidoptera: Geometridae).
Journal of Economic Entomology. Roč. 111, č. 3 (2018), s. 1411-1419. ISSN 0022-0493. E-ISSN 1938-291X
Institucionální podpora: RVO:60077344
Klíčová slova: Ectropis oblique * transcriptome * alimentary tracts
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 1.779, rok: 2018

Ectropis oblique Prout (Lepidoptera: Geometridae) is one of the main pests that damages the tea crop in Southeast Asia. To understand the molecular mechanisms of its feeding biology, transcriptomes of the alimentary tract (AT) and of the body minus the AT of E. oblique were successfully sequenced and analyzed in this study. A total of 36,950 unigenes from de novo sequences were assembled. After analysis using six annotation databases (e.g., Gene Ontology, Kyoto Encyclopedia of Genes and Genome, and NCBI nr), a series of putative genes were found for this insect species that were related to digestion, detoxification, the immune system, and Bacillus thuringiensis (Bt) receptors. From this series of genes, 21 were randomly selected to verify the relative expression levels of transcripts using quantitative real-time polymerase chain reaction. These results will provide an invaluable genomic ressource for future studies on the molecular mechanisms of E. oblique, which will be useful in developing biological kontrol strategies for this pest.
Trvalý link: http://hdl.handle.net/11104/0292611