Počet záznamů: 1
Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels
- 1.0489869 - FGÚ 2019 RIV NL eng J - Článek v odborném periodiku
Lišková, Jana - Douglas, T.E.L. - Wijnants, R. - Samal, S. K. - Mendez, A. C. - Chronakis, I. - Bačáková, Lucie - Skirtach, A. G.
Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels.
Materials Letters. Roč. 214, Mar 1 (2018), s. 186-189. ISSN 0167-577X. E-ISSN 1873-4979
Grant CEP: GA MZd(CZ) NV15-33018A
Institucionální podpora: RVO:67985823
Klíčová slova: biomaterials * biomimetic * composite materials
Obor OECD: Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions (pharmacogenomics, gene-based therapeutics)
Impakt faktor: 3.019, rok: 2018
Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.
Trvalý link: http://hdl.handle.net/11104/0284293
Počet záznamů: 1