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Biodegradation of phenol and its derivatives by engineered bacteria: current knowledge and perspectives
- 1.0489597 - MBÚ 2019 RIV US eng J - Článek v odborném periodiku
Rucká, Lenka - Nešvera, Jan - Pátek, Miroslav
Biodegradation of phenol and its derivatives by engineered bacteria: current knowledge and perspectives.
World Journal of Microbiology and Biotechnology. Roč. 33, č. 9 (2017), č. článku 174. ISSN 0959-3993. E-ISSN 1573-0972
Grant CEP: GA TA ČR(CZ) TA04021212
Institucionální podpora: RVO:61388971
Klíčová slova: Adaptive evolution * Genome shuffling * In situ bioremediation
Obor OECD: Microbiology
Impakt faktor: 2.100, rok: 2017
Biodegradation of phenolic compounds is a promising alternative to physical and chemical methods used to remove these toxic pollutants from the environment. The ability of various microorganisms to metabolize phenol and its derivatives (alkylphenols, nitrophenols and halogenated derivatives) has therefore been intensively studied. Knowledge of the enzymes catalyzing the individual reactions, the genes encoding these enzymes and the regulatory mechanisms involved in the expression of the respective genes in bacteria serves as a basis for the development of more efficient degraders of phenols via genetic engineering methods. Engineered bacteria which efficiently degrade phenolic compounds were constructed in laboratories using various approaches such as cloning the catabolic genes in multicopy plasmids, the introduction of heterologous genes or broadening the substrate range of key enzymes by mutagenesis. Efforts to apply the engineered strains in in situ bioremediation are problematic, since engineered strains often do not compete successfully with indigenous microorganisms. New efficient degraders of phenolic compounds may be obtained by complex approaches at the organism level, such as genome shuffling or adaptive evolution. The application of these engineered bacteria for bioremediation will require even more complex analysis of both the biological characteristics of the degraders and the physico-chemical conditions at the polluted sites.
Trvalý link: http://hdl.handle.net/11104/0283981
Název souboru Staženo Velikost Komentář Verze Přístup 2017 Rucká fenol review WJMB.pdf 5 732.7 KB Autorský preprint vyžádat
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