Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A-glycerinitolerans, and A-ruminicola

0489549 - ÚŽFG 2019 RIV CZ eng J - Článek v odborném periodiku
Šimůnek, Jiří Jr. - Killer, Jiří - Sechovcová, Hana - Šimůnek, Jiří - Pechar, R. - Rada, V. - Švec, P. - Sedláček, I.
Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A-glycerinitolerans, and A-ruminicola.
Folia Microbiologica. Roč. 63, č. 3 (2018), s. 391-399. ISSN 0015-5632. E-ISSN 1874-9356
Grant CEP: GA ČR(CZ) GA16-12431S
Institucionální podpora: RVO:67985904
Klíčová slova: bacterium C10 * red deer
Obor OECD: Microbiology
Impakt faktor: 1.448, rok: 2018

Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic. The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation. The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4(T) and Actinomyces glycerinitolerans G10(T) as the closest relatives followed by the Actinomyces ruminicola DSM 27982(T). Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA and rplB gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains. Thus, gene identities revealed that the evaluated isolate C10 is a little more related to the species A. succiniciruminis isolated from the rumen of a Holstein-Friesian cow than A. glycerinitolerans. Phylogenetic analyses confirmed affinity of strain C10 to both recently characterized species. Unfortunately, they did not allow the bacterial strain to be classified into a particular species. Phenotypic characterization suggested similar conclusions. This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
Trvalý link: http://hdl.handle.net/11104/0283946