Potential of the strain Raoultella sp KDF8 for removal of analgesics

0489382 - MBÚ 2019 RIV NL eng J - Článek v odborném periodiku
Palyzová, Andrea - Zahradník, Jiří - Marešová, Helena - Sokolová, Lucie - Kyslíková, Eva - Grulich, Michal - Štěpánek, Václav - Řezanka, Tomáš - Kyslík, Pavel
Potential of the strain Raoultella sp KDF8 for removal of analgesics.
Folia Microbiologica. Roč. 63, č. 3 (2018), s. 273-282. ISSN 0015-5632. E-ISSN 1874-9356
Grant CEP: GA TA ČR TH02030337
Institucionální podpora: RVO:61388971 ; RVO:86652036
Klíčová slova: Microbial degradation * Raoultella sp. * Analgesics
Obor OECD: Microbiology; Microbiology (BTO-N)
Impakt faktor: 1.448, rok: 2018

The bacterial strain KDF8 capable of growth in the presence of diclofenac and codeine analgesics was obtained after chemical mutagenesis of nature isolates from polluted soils. The strain KDF8 was identified as Raoultella sp. based on its morphology, biochemical properties, and 16S rRNA gene sequence. It was deposited in the Czech Collection of Microorganisms under the number CCM 8678. A growing culture efficiently removed diclofenac (92% removal) and partially also codeine (about 30% degradation) from culture supernatants within 72 h at 28 degrees C. The degradation of six analgesics by the whole cell catalyst was investigated in detail. The maximum degradation of diclofenac (91%) by the catalyst was achieved at pH(INI) of 7 (1 g/L diclofenac). The specific removal rate at high concentrations of diclofenac and codeine increased up to 16.5 mg/g(CDW) per h and 5.1 mg/g(CDW) per h, respectively. HPLC analysis identified 4'-hydroxydiclofenac as a major metabolite of diclofenac transformation and 14-hydroxycodeinone as codeine transformation product. The analgesics ibuprofen and ketoprofen were also removed, albeit to a lower extent of 3.2 and 2.0 mg/g(CDW) per h, respectively. Naproxen and mefenamic acid were not degraded.
Trvalý link: http://hdl.handle.net/11104/0283798