Osmotolerance of Dekkera bruxellensis and the role of two Stl glycerol–proton symporters

0489305 - FGÚ 2019 RIV NL eng J - Článek v odborném periodiku
Zemančíková, Jana - Dušková, Michala - Elicharová, Hana - Papoušková, Klára - Sychrová, Hana
Osmotolerance of Dekkera bruxellensis and the role of two Stl glycerol–proton symporters.
FEMS Microbiology Letters. Roč. 365, č. 5 (2018), č. článku fny020. ISSN 0378-1097. E-ISSN 1574-6968
Grant CEP: GA ČR(CZ) GA15-03708S
Institucionální podpora: RVO:67985823
Klíčová slova: glycerol uptake * osmotolerance * non-conventional yeasts
Obor OECD: Microbiology
Impakt faktor: 1.994, rok: 2018

Dekkera bruxellensis is important for lambic beer fermentation but is considered a spoilage yeast in wine fermentation. We compared two D. bruxellensis strains isolated from wine and found that they differ in some basic properties, including osmotolerance. The genomes of both strains contain two highly similar copies of genes encoding putative glycerol–proton symporters from the STL family that are important for yeast osmotolerance. Cloning of the two DbSTL genes and their expression in suitable osmosensitive Saccharomyces cerevisiae mutants revealed that both identified genes encode functional glycerol uptake systems, but only DbStl2 has the capacity to improve the osmotolerance of S. cerevisiae cells.
Trvalý link: http://hdl.handle.net/11104/0283748