First multigene analysis of Archamoebae (Amoebozoa: Conosa) robustly reveals its phylogeny and shows that Entamoebidae represents a deep lineage of the group

0486726 - ÚMG 2018 RIV US eng J - Článek v odborném periodiku
Panek, T. - Zadrobilkova, E. - Walker, G. - Brown, M. W. - Gentekaki, E. - Hroudová, Miluše - Kang, S. - Roger, A. J. - Tice, A. K. - Vlček, Čestmír - Čepička, I.
First multigene analysis of Archamoebae (Amoebozoa: Conosa) robustly reveals its phylogeny and shows that Entamoebidae represents a deep lineage of the group.
Molecular Phylogenetics and Evolution. Roč. 98, jaro (2016), s. 41-51. ISSN 1055-7903. E-ISSN 1095-9513
Institucionální podpora: RVO:68378050
Klíčová slova: Conosa * Pelobiontida * Evolution of parasitism * Nitrogen fixation system * Flagellar apparatus * Classification
Obor OECD: Microbiology
Impakt faktor: 4.419, rok: 2016

Archamoebae is an understudied group of anaerobic free-living or endobiotic protists that constitutes the major anaerobic lineage of the supergroup Amoebozoa. Hitherto, the phylogeny of Archamoebae was based solely on SSU rRNA and actin genes, which did not resolve relationships among the main lineages of the group. Because of this uncertainty, several different scenarios had been proposed for the phylogeny of the Archamoebae. In this study, we present the first multigene phylogenetic analysis that includes members of Pelomyxidae, and Rhizomastixidae. The analysis clearly shows that Mastigamoebidae, Pelomyxidae and Rhizomastixidae form a Glade of mostly free-living, amoeboid flagellates, here called Pelobiontida. The predominantly endobiotic and aflagellated Entamoebidae represents a separate, deep-branching lineage, Entamoebida. Therefore, two unique evolutionary events, horizontal transfer of the nitrogen fixation system from bacteria and transfer of the sulfate activation pathway to mitochondria] derivatives, predate the radiation of recent lineages of Archamoebae. The endobiotic lifestyle has arisen at least three times independently during the evolution of the group. We also present new ultra structural data that clarifies the primary divergence among the family Mastigamoebidae which had previously been inferred from phylogenetic analyses based on SSU rDNA. (C) 2016 Elsevier Inc. All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0281463