Fab antibody fragment-functionalized liposomes for specific targeting of antigen-positive cells

0486725 - ÚMG 2018 RIV US eng J - Článek v odborném periodiku
Ohradanova-Repic, A. - Nogueira, E. - Hartl, I. - Gomes, A.C. - Preto, A. - Steinhuber, E. - Muehlgrabner, V. - Repic, M. - Kuttke, M. - Zwirzitz, A. - Prouza, M. - Suchánek, M. - Wozniak-Knopp, G. - Hořejší, Václav - Schabbauer, G. - Cavaco-Paulo, A. - Stockinger, H.
Fab antibody fragment-functionalized liposomes for specific targeting of antigen-positive cells.
Nanomedicine: Nanotechnology, Biology and Medicine. Roč. 14, č. 1 (2018), s. 123-130. ISSN 1549-9634. E-ISSN 1549-9642
Institucionální podpora: RVO:68378050
Klíčová slova: Active targeting * Liposome functionalization * Immunoliposome * Antibody engineering * Recombinant Fab antibody fragment
Obor OECD: Cell biology
Impakt faktor: 5.570, rok: 2018

Liposomes functionalized with monoclonal antibodies or their antigen-binding fragments have attracted much attention as specific drug delivery devices for treatment of various diseases including cancer. The conjugation of antibodies to liposomes is usually achieved by covalent coupling using cross-linkers in a reaction that might adversely affect the characteristics of the final product. Here we present an alternative strategy for liposome functionalization: we created a recombinant Fab antibody fragment genetically fused on its C-terminus to the hydrophobic peptide derived from pulmonary surfactant protein D, which became inserted into the liposomal bilayer during liposomal preparation and anchored the Fab onto the liposome surface. The Fab-conjugated liposomes specifically recognized antigen-positive cells and efficiently delivered their cargo, the Alexa Fluor 647 dye, into target cells in vitro and in vivo. In conclusion, our approach offers the potential for straightforward development of nanomedicines functionalized with an antibody of choice without the need of harmful cross-linkers. (C) 2017 Elsevier Inc. All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0281462