Adhesion and differentiation of Saos-2 osteoblast-like cells on chromium-doped diamond-like carbon coatings

0486146 - FGÚ 2018 RIV GB eng J - Článek v odborném periodiku
Filová, Elena - Vandrovcová, Marta - Jelínek, Miroslav - Zemek, Josef - Houdková, Jana - Remsa, Jan - Kocourek, Tomáš - Staňková, Ľubica - Bačáková, Lucie
Adhesion and differentiation of Saos-2 osteoblast-like cells on chromium-doped diamond-like carbon coatings.
Journal of Materials Science-Materials in Medicine. Roč. 28, č. 1 (2017), č. článku 17. ISSN 0957-4530. E-ISSN 1573-4838
Grant CEP: GA ČR(CZ) GA15-05864S; GA ČR(CZ) GA14-04790S; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:67985823 ; RVO:68378271
Klíčová slova: osteocalcin * osteogenic differentiation * hexavalent chromium * focal adhesion contact * cell spreading area
Obor OECD: Biomaterials (as related to medical implants, devices, sensors)
Impakt faktor: 2.448, rok: 2017

Diamond-like carbon (DLC) thin films are promising for use in coating orthopaedic, dental and cardiovascular implants. The problem of DLC layers lies in their weak layer adhesion to metal implants. Chromium is used as a dopant for improving the adhesion of DLC films. Cr-DLC layers were prepared by a hybrid technology, using a combination of pulsed laser deposition (PLD) from a graphite target and magnetron sputtering. Depending on the deposition conditions, the concentration of Cr in the DLC layers moved from zero to 10.0 at.%. The effect of DLC layers with 0.0, 0.9, 1.8, 7.3, 7.7 and 10.0 at.% Cr content on the adhesion and osteogenic differentiation of human osteoblast-like Saos-2 cells was assessed in vitro. The DLC samples that contained 7.7 and 10.0 at.% of Cr supported cell spreading on day 1 after seeding. On day three after seeding, the most apparent vinculin-containing focal adhesion plaques were also found on samples with higher concentrations of chromium. On the other hand, the expression of type I collagen and alkaline phosphatase at the mRNA and protein level was the highest on Cr-DLC samples with a lower concentration of Cr (0-1.8 at.%). We can conclude that higher concentrations of chromium supported cell adhesion, however DLC and DLC doped with a lower concentration of chromium supported osteogenic cell differentiation.
Trvalý link: http://hdl.handle.net/11104/0281008