Distinguishing glycan isomers by voltammetry. Modification of 2,3-sialyllactose and 2,6-sialyllactose by osmium(VI) complexes

0486056 - BFÚ 2018 RIV NL eng J - Článek v odborném periodiku
Trefulka, Mojmír - Paleček, Emil
Distinguishing glycan isomers by voltammetry. Modification of 2,3-sialyllactose and 2,6-sialyllactose by osmium(VI) complexes.
Electrochemistry Communications. Roč. 85, DEC2017 (2017), s. 19-22. ISSN 1388-2481. E-ISSN 1873-1902
Grant CEP: GA ČR(CZ) GA15-15479S
Institucionální podpora: RVO:68081707
Klíčová slova: prostate-specific antigen * maldi-tof-ms * os(vi)-modified polysaccharides
Obor OECD: Analytical chemistry
Impakt faktor: 4.660, rok: 2017

Carbohydrate components in glycoproteins play a critical role in health and disease and specificity of glycoprotein biomarkers can be greatly enhanced by analysis of their sugar components containing frequently different isomers. We show that two glycan isomers, 2,3-sialyllactose and 2,6-sialyllactose (important in cancer), can be distinguished voltammetrically after their modification with osmium(VI) complexes. Using SWV at different frequencies and CV at different scan rates we found conditions for simple discrimination between these isomers at mercury and carbon electrodes.
Trvalý link: http://hdl.handle.net/11104/0280944