Methodological standards for in vitro models of epilepsy and epileptic seizures. A TASK1-WG4 report of the AES/ILAE Translational Task Force of the ILAE

0485938 - FGÚ 2018 RIV US eng J - Článek v odborném periodiku
Raimondo, J. V. - Heinemann, U. - de Curtis, M. - Goodkin, H. P. - Dulla, Ch. G. - Janigro, D. - Ikeda, A. - Lin, Ch.-Ch. K. - Jiruška, Přemysl - Galanopoulou, A. S. - Bernard, Ch.
Methodological standards for in vitro models of epilepsy and epileptic seizures. A TASK1-WG4 report of the AES/ILAE Translational Task Force of the ILAE.
Epilepsia. Roč. 58, Suppl.4 (2017), s. 40-52. ISSN 0013-9580. E-ISSN 1528-1167
Grant CEP: GA MZd(CZ) NV15-29835A; GA MZd(CZ) NV15-33115A; GA ČR(CZ) GA14-02634S; GA ČR(CZ) GA15-08565S
Institucionální podpora: RVO:67985823
Klíčová slova: brain slice preparation * electrophysiological recording methods * recording solution composition * in vitro models of seizures * animal selection and killing
Obor OECD: Neurosciences (including psychophysiology
Impakt faktor: 5.067, rok: 2017

In vitro preparations are a powerful tool to explore the mechanisms and processes underlying epileptogenesis and ictogenesis. In this review, we critically review the numerous invitro methodologies utilized in epilepsy research. We provide support for the inclusion of detailed descriptions of techniques, including often ignored parameters with unpredictable yet significant effects on study reproducibility and outcomes. In addition, we explore how recent developments in brain slice preparation relate to their use as models of epileptic activity.
Trvalý link: http://hdl.handle.net/11104/0280854