Počet záznamů: 1
Satellite DNA and Transposable Elements in Seabuckthorn (Hippophae rhamnoides), a Dioecious Plant with Small Y and Large X Chromosomes
- 1.0485763 - BFÚ 2018 RIV GB eng J - Článek v odborném periodiku
Puterova, Janka - Razumova, O. - Martínek, T. - Alexandrov, O. - Divashuk, M. - Kubát, Zdeněk - Hobza, Roman - Karlov, G. - Kejnovský, Eduard
Satellite DNA and Transposable Elements in Seabuckthorn (Hippophae rhamnoides), a Dioecious Plant with Small Y and Large X Chromosomes.
Genome Biology and Evolution. Roč. 9, č. 1 (2017), s. 197-212. ISSN 1759-6653. E-ISSN 1759-6653
Grant CEP: GA ČR GBP501/12/G090
Grant ostatní: GA MŠk(CZ) LM2010005
Institucionální podpora: RVO:68081707
Klíčová slova: sex-chromosomes * repetitive sequences * silene-latifolia
Obor OECD: Developmental biology
Impakt faktor: 3.940, rok: 2017
Seabuckthorn (Hippophae rhamnoides) is a dioecious shrub commonly used in the pharmaceutical, cosmetic, and environmental industry as a source of oil, minerals and vitamins. In this study, we analyzed the transposable elements and satellites in its genome. We carried out Illumina DNA sequencing and reconstructed the main repetitive DNA sequences. For data analysis, we developed a new bioinformatics approach for advanced satellite DNA analysis and showed that about 25% of the genome consists of satellite DNA and about 24% is formed of transposable elements, dominated by Ty3/Gypsy and Ty1/Copia LTR retrotransposons. FISH mapping revealed X chromosome-accumulated, Y chromosome-specific or both sex chromosomes-accumulated satellites but most satellites were found on autosomes. Transposable elements were located mostly in the subtelomeres of all chromosomes. The 5S rDNA and 45S rDNA were localized on one autosomal locus each. Although we demonstrated the small size of the Y chromosome of the seabuckthorn and accumulated satellite DNA there, we were unable to estimate the age and extent of the Y chromosome degeneration. Analysis of dioecious relatives such as Shepherdia would shed more light on the evolution of these sex chromosomes.
Trvalý link: http://hdl.handle.net/11104/0280706
Počet záznamů: 1