The common lavender (Lavandula angustifolia Mill.) pectic polysaccharides modulate phagocytic leukocytes and intestinal Peyer's patch cells

0485443 - BFÚ 2018 RIV GB eng J - Článek v odborném periodiku
Georgiev, Y.N. - Paulsen, B.S. - Kiyohara, H. - Číž, Milan - Ognyanov, M.H. - Vašíček, Ondřej - Rise, F. - Denev, P. - Yamada, H. - Lojek, Antonín - Kussovski, V. - Barsett, H. - Krastanov, A.I. - Yanakieva, I.Z. - Kratchanova, M.
The common lavender (Lavandula angustifolia Mill.) pectic polysaccharides modulate phagocytic leukocytes and intestinal Peyer's patch cells.
Carbohydrate Polymers. Roč. 174, oct2017 (2017), s. 948-959. ISSN 0144-8617. E-ISSN 1879-1344
Institucionální podpora: RVO:68081707
Klíčová slova: Lavender * Pectin * Immunomodulation
Obor OECD: Cell biology
Impakt faktor: 5.158, rok: 2017

Two pectic (chPS-L1, chPS-L2) and one polyphenolic (chPP-L) fractions were obtained from lavender flowers after boiling water extraction, exhaustive removing of alcohol-soluble molecules and SEC. chPS-L1 (52.4 kDa) contains mainly low-acetylated and high-methoxylated homogalacturonans (HG), and smaller rhamnogalacturonan (RG) I backbone fragments rich in 1,3,5-branched arabinan and arabinogalactan (AG) II side chains. chPS-L2 (21.8 kDa) contains predominantly similarly esterified HG, followed by RGI with AGII structures and RGII. The prevalence of catechin and epicatechin in chPP-L indicates that they form weak interactions with pectins. chPS-L1 and chPS-L2 enhanced beta(2)-integrin expression on neutrophils, inducing ROS generation and macrophage NO production. Both the effects on beta(2)-integrin and high complement fixation activity of chPS-L1 were proposed for its inhibitory action against PMA-and OZP-activated ROS formation. This, together with suppression of NO generation after co-stimulation with chPS-L1 and LPS, suggested anti-inflammatory activity of studied pectins. Lavender polysaccharides expressed intestinal Peyer's patch immunomodulating activity. (C) 2017 Elsevier Ltd. All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0280472