Tilia tomentosa pectins exhibit dual mode of action on phagocytes as beta-glucuronic acid monomers are abundant in their rhamnogalacturonans I

0485442 - BFÚ 2018 RIV GB eng J - Článek v odborném periodiku
Georgiev, Y.N. - Paulsen, B.S. - Kiyohara, H. - Číž, Milan - Ognyanov, M.H. - Vašíček, Ondřej - Rise, F. - Denev, P. - Lojek, Antonín - Batsalova, T.G. - Dzhambazov, B.M. - Yamada, H. - Lund, R. - Barsett, H. - Krastanov, A.I. - Yanakieva, I.Z. - Kratchanova, M.
Tilia tomentosa pectins exhibit dual mode of action on phagocytes as beta-glucuronic acid monomers are abundant in their rhamnogalacturonans I.
Carbohydrate Polymers. Roč. 175, NOV2017 (2017), s. 178-191. ISSN 0144-8617. E-ISSN 1879-1344
Grant ostatní: GA MŠk(CZ) LQ1605
Institucionální podpora: RVO:68081707
Klíčová slova: biophytum-petersianum klotzsch * nitric-oxide production
Obor OECD: Physical chemistry
Impakt faktor: 5.158, rok: 2017

Silver linden flowers contain different pectins (PSI-PSIII) with immunomodulating properties. PSI is a low-esterified pectic polysaccharide with predominant homogalacturonan region, followed by rhamnogalacturonan I (RGI) with arabinogalactan II and RGII (traces) domains. PSII and PSIII are unusual glucuronidated RGI polymers. PSIII is a unique high molecular weight RGI, having almost completely 0-3 glucuronidated GalA units with >30% 0-3 acetylation at the Rha units. Linden pectins induced reactive oxygen species (ROS) and NO generation from non-stimulated whole blood phagocytes and macrophages, resp., but suppressed OZP-(opsonized zymosan particles)-activated ROS generation, LPS-induced iNOS expression and NO production. This dual mode of action suggests their anti-inflammatory activity, which is known for silver linden extracts. PSI expressed the highest complement fixation and macrophage stimulating activities and was active on intestinal Peyer's patch cells. PSIII was active on non-stimulated neutrophils, as it induced beta(2)-integrin expression, revealing that acetylated and highly glucuronidated RGI exhibits immunomodulating properties via phagocytes. (C) 2017 Elsevier Ltd. All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0280469