Genome sequencing reveals metabolic and cellular interdependence in an amoeba-kinetoplastid symbiosis

0485317 - BC 2018 RIV GB eng J - Článek v odborném periodiku
Tanifuji, G. - Cenci, U. - Moog, D. - Dean, S. - Nakayama, T. - David, Vojtěch - Fiala, Ivan - Curtis, B.A. - Sibbald, S. J. - Onodera, N. T. - Colp, M. - Flegontov, Pavel - Johnson-MacKinnon, J. - McPhee, M. - Inagaki, Y. - Hashimoto, T. - Kelly, S. - Gull, K. - Lukeš, Julius - Archibald, J.M.
Genome sequencing reveals metabolic and cellular interdependence in an amoeba-kinetoplastid symbiosis.
Scientific Reports. Roč. 7, SEP 15 (2017), č. článku 11688. ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA ČR(CZ) GA14-23986S; GA MŠMT LL1601
Institucionální podpora: RVO:60077344
Klíčová slova: trypanosoma-brucei reveals * hidden markov model * neoparamoeba-pemaquidensis * gill disease * phylogenetic analyses * ichthyobodo-necator * gene prediction * host control * evolution * proteomics
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 4.122, rok: 2017

Endosymbiotic relationships between eukaryotic and prokaryotic cells are common in nature. Endosymbioses between two eukaryotes are also known, cyanobacterium-derived plastids have spread horizontally when one eukaryote assimilated another. A unique instance of a non-photosynthetic, eukaryotic endosymbiont involves members of the genus Paramoeba, amoebozoans that infect marine animals such as farmed fish and sea urchins. Paramoeba species harbor endosymbionts belonging to the Kinetoplastea, a diverse group of flagellate protists including some that cause devastating diseases. To elucidate the nature of this eukaryote-eukaryote association, we sequenced the genomes and transcriptomes of Paramoeba pemaquidensis and its endosymbiont Perkinsela sp. The endosymbiont nuclear genome is similar to 9.5 Mbp in size, the smallest of a kinetoplastid thus far discovered. Genomic analyses show that Perkinsela sp. has lost the ability to make a flagellum but retains hallmark features of kinetoplastid biology, including polycistronic transcription, trans-splicing, and a glycosome-like organelle. Mosaic biochemical pathways suggest extensive 'cross-talk' between the two organisms, and electron microscopy shows that the endosymbiont ingests amoeba cytoplasm, a novel form of endosymbiont-host communication. Our data reveal the cell biological and biochemical basis of the obligate relationship between Perkinsela sp. and its amoeba host, and provide a foundation for understanding pathogenicity determinants in economically important Paramoeba.
Trvalý link: http://hdl.handle.net/11104/0280383