Application of Raman spectroscopy to analyse lignin/cellulose ratio in Norway spruce tree rings

0485067 - ÚVGZ 2019 RIV CZ eng J - Článek v odborném periodiku
Vítek, Petr - Klem, Karel - Urban, Otmar
Application of Raman spectroscopy to analyse lignin/cellulose ratio in Norway spruce tree rings.
Beskydy. Roč. 20, 1-2 (2017), s. 41-48. ISSN 1803-2451
Grant CEP: GA MŠMT(CZ) LO1415
Institucionální podpora: RVO:86652079
Klíčová slova: dendrochronology * vibrational spectroscopy * lignification * Norway spruce * abiotic stress
Obor OECD: Plant sciences, botany

Tree cores of Picea abies trees (>75 years old) from two different elevations (400 and
1100 m, a. s.l.) in the Jeseníky Mountains were examined. Raman spectroscopy was
used to analyse lignin/cellulose ratio in the latewood of individual tree rings from the
last 35 years. The ratio was calculated based on the Raman intensity of the lignin band
at ~1600 cm–1 assigned to phenyl groups and cellulose band at ~1096 cm–1 based on
a vibration of glycosidic bonds. The results show a clear difference in lignin/cellulose
ratios in trees from high and low elevations, while similar trends in lignin/cellulose
ratios were found in tree cores originating from the same tree but different directions.
Higher lignin/cellulose ratios were found at high elevation as compared to low elevation.
The wood of spruces grown at high elevation also exhibited greater variability of lignin/
cellulose ratios among individual tree rings as compared to trees from low elevation.
A negative correlation between lignin/cellulose ratio and mean annual temperature
was revealed. A weak positive correlation between lignin/cellulose ratio and total
annual precipitation was also found. Redundancy analysis (RDA) showed a pronounced
influence of precipitation in June. The results show great potential for Raman
spectroscopy in tree-ring analysis.
Trvalý link: http://hdl.handle.net/11104/0280171